EBV lysate (TPA induced B95-8 cells)

EL-B-195

EBV VIRAL LYSATE

 

Catalog # EL-B-195                                                   Size 1.0 mg

PRODUCT DESCRIPTION

The EBV (Epstein Barr Virus) is induced in EBV transformed marmoset leukocytes (B-95-8. The cells are pelleted at day 3 after induction (~40% VCA positive) and the pellet is solubilized in 0.1% Triton X-100 and 0.1% SDC and characterized at the biochemical and immunologic levels to assure product consistency and performance.

PRODUCT COMPOSITION

The cell extract is subjected to in vitro testing to ensure lack of infectivity. An infectivity assay is performed to indicate greater than 106 logs of kill for each lot of purified virus. The total protein concentration is determined by Pierce BCA assay.

RECOMMENDED USES

Immunodetection of antibodies to EBV using solid-phase enzyme immunoassay (EIA), WIB, dot-blotting and related techniques. The material could also be used for the purification of viral proteins.

STORAGE AND STABILITY

The viral extract is stable for at least one year when stored at –60oC or below. To avoid freeze-thawing, unused material should be stored in aliquots, preferably under nitrogen in order to prevent oxidation.

AVAILABILITY

Distributed in vials containing 1 mg of protein, shipped on dry ice. Protein concentration ranges from 1.0 to 1.5 mg/ml.

Custom orders are available, including specific buffer formulations and package sizes.

 

All products are for Research Use Only and not for in vitro Diagnostic Use

This Package Insert is provided for product evaluation purposes only and is not intended to be used

in place of the Package Insert shipped with the product.

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